Single-Cell Chromatin and Gene-Regulatory Dynamics of Mouse Nephron Progenitors.

ABSTRACT:

Background

We reasoned that unraveling the dynamic changes in accessibility of genomic regulatory elements and gene expression at single-cell resolution will inform the basic mechanisms of nephrogenesis.

Methods

We performed single-cell ATAC-seq and RNA-seq both individually (singleomes; Six2^GFP cells) and jointly in the same cells (multiomes; kidneys) to generate integrated chromatin and transcriptional maps in mouse embryonic and neonatal nephron progenitor cells.

Results

We demonstrate that singleomes and multiomes are comparable in assigning most cell states, identification of new cell type markers, and defining the transcription factors driving cell identity. However, multiomes are more precise in defining the progenitor population. Multiomes identifie

SUBMITTER: Hilliard S 

PROVIDER: S-EPMC9257825 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature