The Immunological Effect of Oxygen Carriers on Normothermic Ex Vivo Liver Perfusion.

ABSTRACT:

Introduction

Normothermic ex vivo liver perfusion (NEVLP) is an organ preservation method that allows liver graft functional assessment prior to transplantation. One key component of normothermic perfusion solution is an oxygen carrier to provide oxygen to the liver to sustain metabolic activities. Oxygen carriers such as red blood cells (RBCs) or hemoglobin-based oxygen carriers have an unknown effect on the liver-resident immune cells during NEVLP. In this study, we assessed the effects of different oxygen carriers on the phenotype and function of liver-resident immune cells.

Methods

Adult Lewis rat livers underwent NEVLP using three different oxygen carriers: human packed RBCs (pRBCs), rat pRBCs, or Oxyglobin (a synthetic hemoglobin-based oxygen carrier). Hourly pe

SUBMITTER: Jennings H 

PROVIDER: S-EPMC9258194 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature