Long noncoding RNA Smyca coactivates TGF-β/Smad and Myc pathways to drive tumor progression.

ABSTRACT:

Background

Metastasis and chemoresistance are major culprits of cancer mortality, but factors contributing to these processes are incompletely understood.

Methods

Bioinformatics methods were used to identify the relations of Smyca expression to clinicopathological features of human cancers. RNA-sequencing analysis was used to reveal Smyca-regulated transcriptome. RNA pull-down and RNA immunoprecipitation were used to examine the binding of Smyca to Smad3/4 and c-Myc/Max. Chromatin immunoprecipitation and chromatin isolation by RNA purification were used to determine the binding of transcription factors and Smyca to various gene loci, respectively. Real-time RT-PCR and luciferase assay were used to examine gene expression levels and promoter activities, respectively. Xenograf

SUBMITTER: Chen HY 

PROVIDER: S-EPMC9258208 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature