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Transcriptomics of the Rooibos (Aspalathus linearis) Species Complex.


ABSTRACT: Rooibos (Aspalathus linearis), widely known as a herbal tea, is endemic to the Cape Floristic Region of South Africa (SA). It produces a wide range of phenolic compounds that have been associated with diverse health promoting properties of the plant. The species comprises several growth forms that differ in their morphology and biochemical composition, only one of which is cultivated and used commercially. Here, we established methodologies for non-invasive transcriptome research of wild-growing South African plant species, including (1) harvesting and transport of plant material suitable for RNA sequencing; (2) inexpensive, high-throughput biochemical sample screening; (3) extraction of high-quality RNA from recalcitrant, polysaccharide- and polyphenol rich plant material; and (4)

SUBMITTER: Stander EA 

PROVIDER: S-EPMC9258316 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature