An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors.

ABSTRACT: Transcription factors (TFs) regulate gene expression by binding to specific consensus motifs within the local chromatin context. The mechanisms by which TFs navigate the nuclear environment as they search for binding sites remain unclear. Here, we used single-molecule tracking and machine-learning-based classification to directly measure the nuclear mobility of the glucocorticoid receptor (GR) in live cells. We revealed two distinct and dynamic low-mobility populations. One accounts for specific binding to chromatin, while the other represents a confinement state that requires an intrinsically disordered region (IDR), implicated in liquid-liquid condensate subdomains. Further analysis showed that the dwell times of both subpopulations follow a power-law distribution, consistent with a broa

SUBMITTER: Garcia DA 

PROVIDER: S-EPMC9258326 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature