Screening of Hydrocarbon-Stapled Peptides for Inhibition of Calcium-Triggered Exocytosis.

ABSTRACT: The so-called primary interface between the SNARE complex and synaptotagmin-1 (Syt1) is essential for Ca²⁺-triggered neurotransmitter release in neuronal synapses. The interacting residues of the primary interface are conserved across different species for synaptotagmins (Syt1, Syt2, Syt9), SNAP-25, and syntaxin-1A homologs involved in fast synchronous release. This Ca²⁺-independent interface forms prior to Ca²⁺-triggering and plays a role in synaptic vesicle priming. This primary interface is also conserved in the fusion machinery that is responsible for mucin granule membrane fusion. Ca²⁺-stimulated mucin secretion is mediated by the SNAREs syntaxin-3, SNAP-23, VAMP8, Syt2, and other proteins. Here, we designed and screened a series of hydrocar

SUBMITTER: Lai Y 

PROVIDER: S-EPMC9258623 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature