TP53 and EGFR Mutational Status in Thymoma: A Genetic Sequencing Study.

ABSTRACT:

Background and objective

Thymoma is a rare malignant tumor that usually with an indolent presentation, which was falsely assumed to be benign previously. The tumor suppressor P53 (TP53) and EGFR gene mutate most frequently in human cancers. We tried to investigate the presence of TP53 and EGFR mutations among thymoma patients referred to an Indonesian referral respiratory hospital and to discuss its potential role in thymoma management and prognosis.

Material and methods

Surgically resected tumor tissues were collected from thymoma patients and then underwent genomic analysis. PCR was performed on the extracted Paraffinized  DNA to amplify exon 6 of TP53 and exons 18, 19, and 21 of EGFR. The evaluation of mutational status was done using direct sequencing and sequence analys

SUBMITTER: Syahruddin E 

PROVIDER: S-EPMC9258671 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature