Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction.

ABSTRACT: Aflatoxin B1 (AFB₁) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of Pixian Douban, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB₁ detection in Pixian Douban is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB₁ in this sample, for which Fe₃O₄ was synthesized via the solvothermal method and then a Fe₃O₄@SiO₂-NH₂ with a core-shell structure was prepared, followed by an AFB₁-aptamer attachment. The validation was

SUBMITTER: Zeng C 

PROVIDER: S-EPMC9258682 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature