Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging.

ABSTRACT:

Background

Anesthetized animal models are used extensively during neurophysiological and behavioral studies despite systemic effects from anesthesia that undermine both accurate interpretation and translation to awake human physiology. The majority of work examining the impact of anesthesia on cerebral blood flow (CBF) has been restricted to before and after measurements with limited spatial resolution.

New method

We used multi-exposure speckle imaging (MESI), an advanced form of laser speckle contrast imaging (LSCI), to characterize the dynamics of isoflurane anesthesia induction on cerebral vasculature and blood flow in the mouse brain.

Results

The large anatomical changes caused by isoflurane are depicted with wide-field imagery and video highlighting the induction

SUBMITTER: Sullender CT 

PROVIDER: S-EPMC9258779 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature