Mob4-dependent STRIPAK involves the chaperonin TRiC to coordinate myofibril and microtubule network growth.

ABSTRACT: Myofibrils of the skeletal muscle are comprised of sarcomeres that generate force by contraction when myosin-rich thick filaments slide past actin-based thin filaments. Surprisingly little is known about the molecular processes that guide sarcomere assembly in vivo, despite deficits within this process being a major cause of human disease. To overcome this knowledge gap, we undertook a forward genetic screen coupled with reverse genetics to identify genes required for vertebrate sarcomere assembly. In this screen, we identified a zebrafish mutant with a nonsense mutation in mob4. In Drosophila, mob4 has been reported to play a role in spindle focusing as well as neurite branching and in planarians mob4 was implemented in body size regulation. In contrast, zebrafish mob4geh mutants are char

SUBMITTER: Berger J 

PROVIDER: S-EPMC9258817 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature