Cell Sorter Cleaning Practices and Their Impact on Instrument Sterility.

ABSTRACT: Cells isolated using electrostatic cell sorters are subsequently evaluated in a variety of in vitro and in vivo applications. Thus, manipulations to the cells during the pre- and post-sort processing as well as when the cells are being analyzed by and passing through the sorter fluidics has the potential to affect the experimental results. There are many variables to consider when seeking to preserve cellular integrity and function during the cell-sorting process. A previous study by the Association of Biomolecular Resource Facilities Flow Cytometry Research Group (FCRG) investigated downstream effects on different cell types as a function of sorting variables such as pressure, nozzle size, and temperature. This multisite study revealed site-to-site variability based on differential gene e

SUBMITTER: Box A 

PROVIDER: S-EPMC9258916 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature