Quantitation of Exosomes and Their MicroRNA Cargos in Frozen Human Milk.

ABSTRACT: We assessed feasibility of analyzing exosomes and microRNA cargos in frozen human milk as a pre-requisite for epidemiological studies of milk exosomes. We collected milk from five mother-preterm infant dyads at 3 time points during postnatal hospital care for storage at -80°C. We purified exosomes by ultracentrifugation, probed marker proteins using immunoblots, assessed size and counts with a nanoparticle tracker, and quantified three microRNAs with quantitative PCR. Positive exosome marker proteins were detectable; β-casein was the only detectable contaminant. Exosome count and size trended to decrease from early to late samples (count: 2.3×10⁹ ± 3.8×10⁹ to 5.6×10⁸ ± 9.7×10⁸ exosomes/mL; size: 117 ± 25 to 92 ± 16 nm). Two microRNAs were detecta

SUBMITTER: Wang H 

PROVIDER: S-EPMC9258983 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature