Ligand-induced transmembrane conformational coupling in monomeric EGFR.

ABSTRACT: Single pass cell surface receptors regulate cellular processes by transmitting ligand-encoded signals across the plasma membrane via changes to their extracellular and intracellular conformations. This transmembrane signaling is generally initiated by ligand binding to the receptors in their monomeric form. While subsequent receptor-receptor interactions are established as key aspects of transmembrane signaling, the contribution of monomeric receptors has been challenging to isolate due to the complexity and ligand-dependence of these interactions. By combining membrane nanodiscs produced with cell-free expression, single-molecule Förster Resonance Energy Transfer measurements, and molecular dynamics simulations, we report that ligand binding induces intracellular conformational changes wi

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC9259572 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature