TRAF3IP3 Is Cleaved by EV71 3C Protease and Exhibits Antiviral Activity.

ABSTRACT: Enterovirus 71 (EV71) is one of the major pathogens of hand, foot, and mouth disease, which poses a major risk to public health and infant safety. 3C protease (3C^pro), a non-structural protein of EV71, promotes viral protein maturation by cleaving polyprotein precursors and facilitates viral immune escape by cleaving host proteins. In this study, we screened for human proteins that could interact with EV71 3C^pro using a yeast two-hybrid assay. Immune-associated protein TRAF3 Interacting Protein 3 (TRAF3IP3) was selected for further study. The results of co-immunoprecipitation and immunofluorescence demonstrated the interaction between TRAF3IP3 and EV71 3C^pro. A cleavage band was detected, indicating that both transfected 3C^pro and EV71 infection

SUBMITTER: Li H 

PROVIDER: S-EPMC9260427 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature