Composition and diversity analysis of the lung microbiome in patients with suspected ventilator-associated pneumonia.

ABSTRACT:

Background

Ventilator-associated pneumonia (VAP) is associated with high morbidity and health care costs, yet diagnosis remains a challenge. Analysis of airway microbiota by amplicon sequencing provides a possible solution, as pneumonia is characterised by a disruption of the microbiome. However, studies evaluating the diagnostic capabilities of microbiome analysis are limited, with a lack of alignment on possible biomarkers. Using bronchoalveolar lavage fluid (BALF) from ventilated adult patients suspected of VAP, we aimed to explore how key characteristics of the microbiome differ between patients with positive and negative BALF cultures and whether any differences could have a clinically relevant role.

Methods

BALF from patients suspected of VAP was analysed using 16s rRN

SUBMITTER: Fenn D 

PROVIDER: S-EPMC9261066 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature