Curation of a reference database of COI sequences for insect identification through DNA metabarcoding: COins.

ABSTRACT: DNA metabarcoding is a widespread approach for the molecular identification of organisms. While the associated wet-lab and data processing procedures are well established and highly efficient, the reference databases for taxonomic assignment can be implemented to improve the accuracy of identifications. Insects are among the organisms for which DNA-based identification is most commonly used; yet, a DNA-metabarcoding reference database specifically curated for their species identification using software requiring local databases is lacking. Here, we present COins, a database of 5' region cytochrome c oxidase subunit I sequences (COI-5P) of insects that includes over 532 000 representative sequences of >106 000 species specifically formatted for the QIIME2 software platform. Through a combin

SUBMITTER: Magoga G 

PROVIDER: S-EPMC9261288 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature