Establishment of indirect competitive enzyme-linked immunosorbent assay for the detection of platycodin D in Radix Platycodonis.

ABSTRACT: Platycodin D (PD) has been used as the quality control marker of Radix Platycodonis for its high content and various pharmacological properties. In this study, a specific polyclonal antibody against PD (PD-pAb) was developed, and PD-pAb-based indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for the detection of PD in Radix Platycodonis. The 50% inhibition concentration (IC₅₀) of PD was 2.70 μg/mL and the linearity range for PD was from 0.032 μg/mL to 100 μg/mL. No cross reactivity with PD-pAb was found in five PD analogs except for platycodin D2 (PD2, 0.93%). The average recovery of PD by icELISA was 97.14% (RSD = 1.17%). The icELISA was used for the detection of PD in different Radix Platycodonis samples and the results were confirmed by high per

SUBMITTER: Luo S 

PROVIDER: S-EPMC9261796 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature