Three-way analysis-based pH-UV-Vis spectroscopy for quantifying allura red in an energy drink and determining colorant's pKa.

ABSTRACT: Three-way analysis-based pH-UV-Vis spectroscopy was proposed for quantifying allura red in an energy drink product without the need for chromatographic analysis, and determining the colorant's pKa without using any titration technique. In this study, UV-Vis spectroscopic data matrices were obtained from absorbance measurements at five different pH levels from pH 8 to pH 12 and arranged as a three-way array (wavelength × sample × pH). In the three-way analysis procedure, parallel factor analysis (PARAFAC) was implemented to decompose the three-way array into a set of trilinear components. Each set of three components relates to spectral, pH and relative concentration profiles of allura red and sample matrix in the energy drink. First, UV-Vis spectra of the colorant's acid-base pair and samp

SUBMITTER: Dinc E 

PROVIDER: S-EPMC9261852 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature