Phase- and state-dependent modulation of breathing pattern by preBotzinger complex somatostatin expressing neurons.

ABSTRACT: As neuronal subtypes are increasingly categorized, delineating their functional role is paramount. The preBötzinger complex (preBötC) subpopulation expressing the neuropeptide somatostatin (SST) is classified as mostly excitatory, inspiratory-modulated and not rhythmogenic. We further characterized their phenotypic identity: 87% were glutamatergic and the balance were glycinergic and/or GABAergic. We then used optogenetics to investigate their modulatory role in both anaesthetized and freely moving mice. In anaesthetized mice, short photostimulation (100 ms) of preBötC SST⁺ neurons modulated breathing-related variables in a combinatory phase- and state-dependent manner; changes in inspiratory duration, inspiratory peak amplitude (Amp), and phase were different at higher (≥2.5 Hz

SUBMITTER: de Sousa Abreu RP 

PROVIDER: S-EPMC9261878 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature