Gasdermin-D activation by SARS-CoV-2 triggers NET and mediate COVID-19 immunopathology.

ABSTRACT:

Background

The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases of COVID-19. However, the molecular mechanisms underlying the release of NETs in COVID-19 remain unclear.

Objectives

We aim to investigate the role of the Gasdermin-D (GSDMD) pathway on NETs release and the development of organ damage during COVID-19.

Methods

We performed a single-cell transcriptome analysis in public data of bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients with moderate and severe COVID-19. We analyze in blood and lung tissue samples the expression of GSDMD, presence of NETs, and signaling pathways upstreaming. Furthermore, we analyzed the treatment with disulfiram in a mouse model of

SUBMITTER: Silva CMS 

PROVIDER: S-EPMC9261892 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature