Isolation of Tobacco Mosaic Virus-Binding Peptides for Biotechnology Applications.

ABSTRACT: Tobacco mosaic virus (TMV) was the first virus to be discovered and it is now widely used as a tool for biological research and biotechnology applications. TMV particles can be decorated with functional molecules by genetic engineering or bioconjugation. However, this can destabilize the nanoparticles, and/or multiple rounds of modification may be necessary, reducing product yields and preventing the display of certain cargo molecules. To overcome these challenges, we used phage display technology and biopanning to isolate a TMV-binding peptide (TBP_T25 ) with strong binding properties (IC₅₀ =0.73 μM, K_D =0.16 μM), allowing the display of model cargos via a single mixing step. The TMV-binding peptide is specific for TMV but does not recognize free coat prote

SUBMITTER: Chan SK 

PROVIDER: S-EPMC9262120 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature