Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials.

ABSTRACT: Nanobodies represent valuable tools in advanced therapeutic strategies but their small size (∼2.5 × ∼ 4 nm) and limited valence for interactions might pose restrictions for in vivo applications, especially regarding their modest capacity for multivalent and cooperative interaction. In this work, modular protein constructs have been designed, in which nanobodies are fused to protein domains to provide further functionalities and to favor oligomerization into stable self-assembled nanoparticles. The nanobody specificity for their targets is maintained in such supramolecular complexes. Also, their diameter around 70 nm and multivalent interactivity should favor binding and penetrability into target cells via solvent-exposed receptor. These concepts have been supported by unrelated nano

SUBMITTER: Sanchez-Garcia L 

PROVIDER: S-EPMC9262252 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature