Predictions and experimental tests of a new biophysical model of the mammalian respiratory oscillator.

ABSTRACT: Previously our computational modeling studies (Phillips et al., 2019) proposed that neuronal persistent sodium current (I_NaP) and calcium-activated non-selective cation current (I_CAN) are key biophysical factors that, respectively, generate inspiratory rhythm and burst pattern in the mammalian preBötzinger complex (preBötC) respiratory oscillator isolated in vitro. Here, we experimentally tested and confirmed three predictions of the model from new simulations concerning the roles of I_NaP and I_CAN: (1) I_NaP and I_CAN blockade have opposite effects on the relationship between network excitability and preBötC rhythmic activity; (2) I_NaP is essential for preBötC rhythmogenesis; and (3) I_CAN is essential for

SUBMITTER: Phillips RS 

PROVIDER: S-EPMC9262387 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature