Guiding the folding of G-quadruplexes through loop residue interactions.

ABSTRACT: A G-rich sequence was designed to allow folding into either a stable parallel or hybrid-type topology. With the parent sequence featuring coexisting species, various related sequences with single and double mutations and with a shortened central propeller loop affected the topological equilibrium. Two simple modifications, likewise introduced separately to all sequences, were employed to lock folds into one of the topologies without noticeable structural alterations. The unique combination of sequence mutations, high-resolution NMR structural information, and the thermodynamic stability for both topological competitors identified critical loop residue interactions. In contrast to first loop residues, which are mostly disordered and exposed to solvent in both propeller and lateral loops bri

SUBMITTER: Jana J 

PROVIDER: S-EPMC9262619 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature