Stitchr: stitching coding TCR nucleotide sequences from V/J/CDR3 information.

ABSTRACT: The study and manipulation of T cell receptors (TCRs) is central to multiple fields across basic and translational immunology research. Produced by V(D)J recombination, TCRs are often only recorded in the literature and data repositories as a combination of their V and J gene symbols, plus their hypervariable CDR3 amino acid sequence. However, numerous applications require full-length coding nucleotide sequences. Here we present Stitchr, a software tool developed to specifically address this limitation. Given minimal V/J/CDR3 information, Stitchr produces complete coding sequences representing a fully spliced TCR cDNA. Due to its modular design, Stitchr can be used for TCR engineering using either published germline or novel/modified variable and constant region sequences. Sequences produc

SUBMITTER: Heather JM 

PROVIDER: S-EPMC9262623 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature