Homeodomain protein HOMEZ is dispensable for male fertility in mice.

ABSTRACT:

Background

Homeodomain (HD) proteins contain an evolutionarily conserved helix-turn-helix (HTH) DNA-binding motif and act as transcription factors to control gene expression. A previous study showed that the HD gene Homez is highly enriched in adult testes. However, the role of HOMEZ in spermatogenesis and male fertility remains unknown.

Methods

Using CRISPR/Cas9 technology, Homez mutant mice were generated and performed histological, immunofluorescence, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blot and mating assays to analyze the phenotype of Homez mutants.

Results

Molecular phylogenetic analyses indicated that the HOMEZ is evolutionarily conserved among mammalian species. qRT-PCR and Western blot analyses

SUBMITTER: Yue Q 

PROVIDER: S-EPMC9262745 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature