The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes.

ABSTRACT:

Background

Blood is a common source of RNA for gene expression studies. However, it is known to be vulnerable to pre-analytical variables. Although RNA stabilization systems have been shown to reduce such influence, traditional EDTA tubes are still widely used since they are less expensive and enable to study specific leukocyte populations. This study aimed to assess the influence of storage time and temperature between blood sampling and handling on RNA from peripheral blood mononuclear cells (PBMCs).

Methods and results

Nine blood samples were collected in EDTA tubes from 10 healthy donors. One tube from each donor was immediately processed for PBMC isolation, while the others were first incubated at either 4 degrees Celsius (°C) or room temperature for 2, 4, 6 and 24 h. R

SUBMITTER: Martire S 

PROVIDER: S-EPMC9262796 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature