Nanoscale organization of two-dimensional multimeric pMHC reagents with DNA origami for CD8⁺ T cell detection.

ABSTRACT: Peptide-MHC (pMHC) multimers have excelled in the detection of antigen-specific T cells and have allowed phenotypic analysis using other reagents, but their use for detection of low-affinity T cells remains a challenge. Here we develop a multimeric T cell identifying reagent platform using two-dimensional DNA origami scaffolds to spatially organize pMHCs (termed as dorimers) with nanoscale control. We show that these dorimers enhance the binding avidity for low-affinity antigen-specific T cell receptors (TCRs). The dorimers are able to detect more antigen-specific T cells in mouse CD8⁺ T cells and early-stage CD4⁺CD8⁺ double-positive thymocytes that express less dense TCRs, compared with the equivalent tetramers and dextramers. Moreover, we demonstrate dori

SUBMITTER: Sun Y 

PROVIDER: S-EPMC9263106 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature