Microneedle Array-Assisted, Direct Delivery of Genome-Editing Proteins Into Plant Tissue.

ABSTRACT: Genome editing in plants employing recombinant DNA often results in the incorporation of foreign DNA into the host genome. The direct delivery of genome-editing proteins into plant tissues is desired to prevent undesirable genetic alterations. However, in most currently available methods, the point of entry of the genome-editing proteins cannot be controlled and time-consuming processes are required to select the successfully transferred samples. To overcome these limitations, we considered a novel microneedle array (MNA)-based delivery system, in which the needles are horizontally aligned from the substrate surface, giving it a comb-like configuration. We aimed to deliver genome-editing proteins directly into the inner layers of leaf tissues; palisade, the spongy and subepidermal L2 layer

SUBMITTER: Viswan A 

PROVIDER: S-EPMC9263851 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature