Nebulization of siRNA for inhalation therapy based on a microfluidic surface acoustic wave platform.

ABSTRACT: The local delivery of therapeutic small interfering RNA or siRNA to the lungs has the potential to improve the prognosis for patients suffering debilitating lung diseases. Recent advances in materials science have been aimed at addressing delivery challenges including biodistribution, bioavailability and cell internalization, but an equally important challenge to overcome is the development of an inhalation device that can deliver the siRNA effectively to the lung, without degrading the therapeutic itself. Here, we report the nebulization of siRNA, either naked siRNA or complexed with polyethyleneimine (PEI) or a commercial transfection agent, using a miniaturizable acoustomicrofluidic nebulization device. The siRNA solution could be nebulised without significant degradation into an aeroso

SUBMITTER: Cortez-Jugo C 

PROVIDER: S-EPMC9263997 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature