Pilot production of a sensitive ELISA kit and an immunochromatographic strip for rapid detecting citrinin in fermented rice.

ABSTRACT: Citrinin (CTN) is a mycotoxin primarily produced by Monascus species. Excess consumption of CTN may lead to nephrotoxicity and hepatotoxicity. A pilot study for commercial production of competitive direct enzyme-linked immunosorbent assay (cdELISA) kit and an immunochromatographic strip (immunostrip) for screening CTN in red yeast rice is established in this study. The coating antibody and the CTN-horse radish peroxidase (HRP) concentrations were optimized to increase the sensitivity and specificity of cdELISA kit. The conjugation methods/ratios of CTN to HRP as well as the long-term stability of kit components were also evaluated. The IC₅₀ and detection limit of the ELISA kit were determined to be 4.1 and 0.2 ng mL^-1, respectively. Analysis of 20 red yeast ric

SUBMITTER: Wu SW 

PROVIDER: S-EPMC9264126 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature