Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging.

ABSTRACT: Tissues and organs are composed of distinct cell types that must operate in concert to perform physiological functions. Efforts to create high-dimensional biomarker catalogs of these cells have been largely based on single-cell sequencing approaches, which lack the spatial context required to understand critical cellular communication and correlated structural organization. To probe in situ biology with sufficient depth, several multiplexed protein imaging methods have been recently developed. Though these technologies differ in strategy and mode of immunolabeling and detection tags, they commonly utilize antibodies directed against protein biomarkers to provide detailed spatial and functional maps of complex tissues. As these promising antibody-based multiplexing approaches become more wi

SUBMITTER: Hickey JW 

PROVIDER: S-EPMC9264278 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature