Bioprinting Decellularized Breast Tissue for the Development of Three-Dimensional Breast Cancer Models.

ABSTRACT: The tumor extracellular matrix (ECM) plays a vital role in tumor progression and drug resistance. Previous studies have shown that breast tissue-derived matrices could be an important biomaterial to recreate the complexity of the tumor ECM. We have developed a method for decellularizing and delipidating a porcine breast tissue (TDM) compatible with hydrogel formation. The addition of gelatin methacrylamide and alginate allows this TDM to be bioprinted by itself with good printability, shape fidelity, and cytocompatibility. Furthermore, this bioink has been tuned to more closely recreate the breast tumor by incorporating collagen type I (Col1). Breast cancer cells (BCCs) proliferate in both TDM bioinks forming cell clusters and spheroids. The addition of Col1 improves the printability of th

SUBMITTER: Blanco-Fernandez B 

PROVIDER: S-EPMC9264314 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature