¹³C tracer analysis suggests extensive recycling of endogenous CO₂ in vivo.

ABSTRACT:

Background

¹³C tracer analysis is increasingly used to monitor cellular metabolism in vivo and in intact cells, but data interpretation is still the key element to unveil the complexity of metabolic activities. The distinct ¹³C labeling patterns (e.g., M + 1 species in vivo but not in vitro) of metabolites from [U-¹³C]-glucose or [U-¹³C]-glutamine tracing in vivo and in vitro have been previously reported by multiple groups. However, the reason for the difference in the M + 1 species between in vivo and in vitro experiments remains poorly understood.

Methods

We have performed [U-¹³C]-glucose and [U-¹³C]-glutamine tracing in sarcoma-bearing mice (in vivo) and in cancer cell lines (in vitro). ¹³C enri

SUBMITTER: Duan L 

PROVIDER: S-EPMC9264524 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature