Tongxinluo-pretreated mesenchymal stem cells facilitate cardiac repair via exosomal transfer of miR-146a-5p targeting IRAK1/NF-κB p65 pathway.

ABSTRACT:

Background

Bone marrow cells (BMCs), especially mesenchymal stem cells (MSCs), have shown attractive application prospects in acute myocardial infarction (AMI). However, the weak efficacy becomes their main limitation in clinical translation. Based on the anti-inflammation and anti-apoptosis effects of a Chinese medicine-Tongxinluo (TXL), we aimed to explore the effects of TXL-pretreated MSCs (MSCs^TXL) in enhancing cardiac repair and further investigated the underlying mechanism.

Methods

MSCs^TXL or MSCs and the derived exosomes (MSCs^TXL-exo or MSCs-exo) were collected and injected into the infarct zone of rat hearts. In vivo, the anti-apoptotic and anti-inflammation effects, and cardiac functional and histological recovery were evaluated. In

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC9264662 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature