Identification of a replication protein and repeats essential for DNA replication of the temperate lactococcal bacteriophage TP901-1.

ABSTRACT: DNA replication of the temperate lactococcal bacteriophage TP901-1 was shown to involve the gene product encoded by orf13 and the repeats located within the gene. Sequence analysis of 1,500 bp of the early transcribed region of the phage genome revealed a single-stranded DNA binding protein analogue (ORF12) and the putative replication protein (ORF13). The putative origin of replication was identified as series of repeats within orf13 and was shown to confer a TP901-1 resistance phenotype when present in trans. Site-specific mutations were introduced into the replication protein and into the repeats. The mutations were introduced into the TP901-1 prophage by homologous recombination by using a vector with a temperature-sensitive replicon. Subsequent analysis of induced phages showed that t

SUBMITTER: Ostergaard S 

PROVIDER: S-EPMC92647 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature