Effects of end-stage osteoarthritis on markers of skeletal muscle Long INterspersed Element-1 activity.

ABSTRACT:

Objective

Long INterspersed Element-1 (L1) is an autonomous transposable element in the genome. L1 transcripts that are not reverse transcribed back into the genome can accumulate in the cytoplasm and activate an inflammatory response via the cyclic GMP-AMP (cGAS)-STING pathway. We examined skeletal muscle L1 markers as well as STING protein levels in 10 older individuals (63 ± 11 y, BMI = 30.2 ± 6.8 kg/m²) with end-stage osteoarthritis (OA) undergoing total hip (THA, n = 4) or knee (TKA, n = 6) arthroplasty versus 10 young, healthy comparators (Y, 22 ± 2 y, BMI = 23.2 ± 2.5 kg/m²). For OA, muscle was collected from surgical (SX) and contralateral (CTL) sides whereas single vastus lateralis samples were collected from Y.

Results

L1 mRNA was higher in CT

SUBMITTER: Osburn SC 

PROVIDER: S-EPMC9264706 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature