Comprehensive Evaluation of Different TiO₂-Based Phosphopeptide Enrichment and Fractionation Methods for Phosphoproteomics.

ABSTRACT: Protein phosphorylation is an essential post-translational modification that regulates multiple cellular processes. Due to their low stoichiometry and ionization efficiency, it is critical to efficiently enrich phosphopeptides for phosphoproteomics. Several phosphopeptide enrichment methods have been reported; however, few studies have comprehensively compared different TiO₂-based phosphopeptide enrichment methods using complex proteomic samples. Here, we compared four TiO₂-based phosphopeptide enrichment methods that used four non-phosphopeptide excluders (glutamic acid, lactic acid, glycolic acid, and DHB). We found that these four TiO₂-based phosphopeptide enrichment methods had different enrichment specificities and that phosphopeptides enriched by the

SUBMITTER: Li J 

PROVIDER: S-EPMC9265536 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature