Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein.

ABSTRACT:

Background

Night-migratory birds sense the Earth's magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin.

Methods

Protein-protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer.

Results

Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoy

SUBMITTER: Gortemaker K 

PROVIDER: S-EPMC9265643 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature