Defining Optimal Conditions for Tumor Extracellular Vesicle DNA Extraction for Mutation Profiling.

ABSTRACT: (1) Background: Extracellular vesicles (EVs) have emerged as crucial players in the communication between cells in both physiological and pathological scenarios. The functions of EVs are strongly determined by their molecular content, which includes all bioactive molecules, such as proteins, lipids, RNA, and, as more recently described, double-stranded DNA. It has been shown that in oncological settings DNA associated with EVs (EV-DNA) is representative of the genome of parental cells and that it reflects the mutational status of the tumor, gaining much attention as a promising source of biomarker mutant DNA. However, one of the challenges in studies of EV-DNA is the lack of standardization of protocols for the DNA extraction from EVs, as well as ways to assess quality control, which hinde

SUBMITTER: Elzanowska J 

PROVIDER: S-EPMC9265681 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature