Hypothalamic CREB Regulates the Expression of Pomc-Processing Enzyme Pcsk2.

ABSTRACT:

Background

The hypothalamic proopiomelanocortin (Pomc) neurons act as first-order sensors of systemic energy stores, providing signals that regulate caloric intake and energy expenditure. In experimental obesity, dietary saturated fatty acids affect Pomc endopeptidases (PCs), resulting in the abnormal production of the neurotransmitters α-melanocyte-stimulating hormone (α-MSH) and β-endorphin, thus impacting energy balance. The cAMP response element-binding protein (CREB) is one of the transcription factors that control the expression of Pomc endopeptidases; however, it was previously unknown if dietary fats could affect CREB and consequently the expression of Pomc endopeptidases.

Methods

Here, we used single-cell RNA sequencing analysis, PCR, immunoblot, ELISA and immunoflu

SUBMITTER: Zanesco AM 

PROVIDER: S-EPMC9265861 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature