Epigenetic Alterations in Cryopreserved Human Spermatozoa: Suspected Potential Functional Defects.

ABSTRACT:

Background

Gene set enrichment analysis (GSEA) was conducted on raw data, and alternative splicing (AS) events were found after mRNA sequencing of human spermatozoa. In this study, we aimed to compare unknown micro-epigenetics alternations in fresh and cryopreserved spermatozoa to evaluate the effectivity of cryopreservation protocols.

Methods

Spermatozoa were divided into three groups: fresh spermatozoa (group 1), cryoprotectant-free vitrified spermatozoa (group 2), and conventionally frozen spermatozoa (group 3). Nine RNA samples (three replicates in each group) were detected and were used for library preparation with an Illumina compatible kit and sequencing by the Illumina platform.

Results

Three Gene Ontology (GO) terms were found to be enriched in vitrified sper

SUBMITTER: Wang W 

PROVIDER: S-EPMC9266127 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature