ROS Signaling Mediates Directional Cell Elongation and Somatic Cell Fusion in the Red Alga Griffithsia monilis.

ABSTRACT: In many filamentous red algae, cells that die from physical damage are replaced through somatic fusion of repair cells formed from adjacent cells. We visualized ROS generation in repair cells of Giriffthsia monilis using DCFH-DA staining and examined the expression of the genes involved in wound healing using quantitative PCR. Repair cells elongate along the H₂O₂ gradient, meet at each other's tips where the H₂O₂ concentration is highest, and undergo somatic fusion. No wound response occurred with ascorbic acid treatment. Conversely, H₂O₂ treatment induced many repair cells, leading to multiple somatic cell fusions. Diphenylene iodonium (DPI) or caffeine treatment reversibly inhibited ROS production in repair cells an

SUBMITTER: Moon JS 

PROVIDER: S-EPMC9266221 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature