Modeling of the Senescence-Associated Phenotype in Human Skin Fibroblasts.

ABSTRACT: Modern understanding of aging is based on the accumulation of cellular damage during one's life span due to the gradual deterioration of regenerative mechanisms in response to the continuous effect of stress, lifestyle, and environmental factors, followed by increased morbidity and mortality. Simultaneously, the number of senescent cells accumulate exponentially as organisms age. Cell culture models are valuable tools to investigate the mechanisms of aging by inducing cellular senescence in stress-induced premature senescence (SIPS) models. Here, we explain the three-step and one-step H₂O₂-induced senescence models of SIPS designed and reproduced on different human dermal fibroblast cell lines (CCD-1064Sk, CCD-1135Sk, and BJ-5ta). In both SIPS models, it was evident t

SUBMITTER: Gerasymchuk M 

PROVIDER: S-EPMC9266450 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature