Construction and Analysis of Disuse Atrophy Model of the Gastrocnemius Muscle in Chicken.

ABSTRACT: Disuse muscle atrophy is identified as the physiological, biochemical, morphological, and functional changes during restricted movement, immobilization, or weightlessness. Although its internal mechanism has been extensively studied in mammals and was thought to be mainly related to oxidative stress, it was unclear whether it behaved consistently in non-mammals such as chickens. In this study, we tried to construct a disuse atrophy model of the gastrocnemius muscle in chickens by limb immobilization, and collected the gastrocnemius muscles of the fixed group and the control group for RNA sequencing. Through analysis of muscle loss, HE staining, immunohistochemistry, and oxidative stress level, we found that limb immobilization could lead to loss of muscle mass, decrease in muscle fiber dia

SUBMITTER: Mo J 

PROVIDER: S-EPMC9266690 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature