CITF1 Functions Downstream of SPL7 to Specifically Regulate Cu Uptake in Arabidopsis.

ABSTRACT: Copper (Cu) is one of the most indispensable micronutrients, and proper Cu homeostasis is required for plants to maintain essential cellular functions. Plants activate the Cu uptake system during Cu limitation. Although SPL7 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 7) and CITF1 (Cu-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1) are two transcription factors in Cu homeostasis, it remains unclear how SPL7 and CITF1 control the Cu uptake system. Here, we reveal that overexpression of CITF1 causes the enhanced tolerance to Cu deficiency and the elevated expression of Cu uptake genes COPT2, FRO4 and FRO5. Electrophoretic mobility shift assays (EMSA) and transient expression assays indicate that SPL7 directly binds to and activates the promoter of CITF1. The overexp

SUBMITTER: Cai Y 

PROVIDER: S-EPMC9266912 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature