Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.

ABSTRACT: The aim of this research was to determine the impact of heat stress on cell differentiation in an equine mesenchymal stem cell model (EMSC) through the application of heat stress to primary EMSCs as they progressed through the cell specialization process. A proteomic analysis was performed using mass spectrometry to compare relative protein abundances among the proteomes of three cell types: progenitor EMSCs and differentiated osteoblasts and adipocytes, maintained at 37 °C and 42 °C during the process of cell differentiation. A cell-type and temperature-specific response to heat stress was observed, and many of the specific differentially expressed proteins were involved in cell-signaling pathways such as Notch and Wnt signaling, which are known to regulate cellular development. Furthermo

SUBMITTER: Abd-El-Aziz A 

PROVIDER: S-EPMC9267023 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature