Computational Prediction and Experimental Validation of the Unique Molecular Mode of Action of Scoulerine.

ABSTRACT: Scoulerine is a natural compound that is known to bind to tubulin and has anti-mitotic properties demonstrated in various cancer cells. Its molecular mode of action has not been precisely known. In this work, we perform computational prediction and experimental validation of the mode of action of scoulerine. Based on the existing data in the Protein Data Bank (PDB) and using homology modeling, we create human tubulin structures corresponding to both free tubulin dimers and tubulin in a microtubule. We then perform docking of the optimized structure of scoulerine and find the highest affinity binding sites located in both the free tubulin and in a microtubule. We conclude that binding in the vicinity of the colchicine binding site and near the laulimalide binding site are the most likely lo

SUBMITTER: Moshari M 

PROVIDER: S-EPMC9268612 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature