Counting Small Particles in Electron Microscopy Images-Proposal for Rules and Their Application in Practice.

ABSTRACT: Electron microscopy (EM) is the gold standard for the characterisation of the morphology (size and shape) of nanoparticles. Visual observation of objects under examination is always a necessary first step in the characterisation process. Several questions arise when undertaking to identify and count particles to measure their size and shape distribution. In addition to challenges with the dispersion and identification of the particles, more than one protocol for counting particles is in use. This paper focuses on precise rules for the counting of particles in EM micrographs, as this influences the measurement accuracy of the number of particles, thus implicitly affecting the size values of the counted particles. We review and compare four different, commonly used methods for counting, whic

SUBMITTER: Bresch H 

PROVIDER: S-EPMC9268650 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature