Copy number variations across the blood-brain barrier in multiple sclerosis.

ABSTRACT:

Objective

Multiple sclerosis (MS) is a neuroinflammatory disease where immune cells cross the blood-brain barrier (BBB) into the central nervous system (CNS). What predisposes these immune cells to cross the BBB is still unknown. Here, we examine the possibility that genomic rearrangements could predisposespecific immune cells in the peripheral blood to cross the BBB and form sub-populations of cells involved in the inflammatory process in the CNS.

Methods

We compared copy number variations in paired peripheral blood mononuclear cells (PBMCs) and cerebrospinal fluid (CSF) cells from MS patients. Thereafter, using next generation sequencing, we studied the T-cell receptor beta (TRB) locus rearrangements and profiled the αβ T cell repertoire in peripheral CD4⁺ and C

SUBMITTER: Bedri SK 

PROVIDER: S-EPMC9268884 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature